Crude fucoidan extracts impair angiogenesis in models relevant for bone regeneration and osteosarcoma via reduction of VEGF and SDF-1

43Citations
Citations of this article
52Readers
Mendeley users who have this article in their library.

Abstract

The marine origin polysaccharide fucoidan combines multiple biological activities. As demonstrated by various studies in vitro and in vivo, fucoidans show anti-viral, anti-tumor, anti-oxidant, anti-inflammatory and anti-coagulant properties, although the detailed molecular action remains to be elucidated. The aim of the present study is to assess the impact of crude fucoidan extracts, on the formation of vascular structures in co-culture models relevant for bone vascularization during bone repair and for vascularization processes in osteosarcoma. The co-cultures consisted of bone marrow derived mesenchymal stem cells, respectively the osteosarcoma cell line MG63, and human blood derived outgrowth endothelial cells (OEC). The concentration dependent effects on the metabolic activity on endothelial cells and osteoblast cells were first assessed using monocultures of OEC, MSC and MG63 suggesting a concentration of 100 μg/mL as a suitable concentration for further experiments. In co-cultures fucoidan significantly reduced angiogenesis in MSC/OEC but also in MG63/OEC co-cultures suggesting a potential application of fucoidan to lower the vascularization in bone tumors such as osteosarcoma. This was associated with a decrease in VEGF (vascular endothelial growth factor) and SDF-1 (stromal derived factor-1) on the protein level, both related to the control of angiogenesis and furthermore discussed as crucial factors in osteosarcoma progression and metastasis. In terms of bone formation, fucoidan slightly lowered on the calcification process in MSC monocultures and MSC/OEC co-cultures. In summary, these data suggest the suitability of lower fucoidan doses to limit angiogenesis for instance in osteosarcoma.

References Powered by Scopus

Inhibition of vascular endothelial growth factor-induced angiogenesis suppresses tumour growth in vivo

3451Citations
N/AReaders
Get full text

Vascularization in tissue engineering

1045Citations
N/AReaders
Get full text

Mechanisms of normal and tumor-derived angiogenesis

677Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Biological activities of fucoidan and the factors mediating its therapeutic effects: A review of recent studies

362Citations
N/AReaders
Get full text

Fucoidan structure and activity in relation to anti-cancer mechanisms

256Citations
N/AReaders
Get full text

Clinical application of bone marrow mesenchymal stem/stromal cells to repair skeletal tissue

180Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Wang, F., Schmidt, H., Pavleska, D., Wermann, T., Seekamp, A., & Fuchs, S. (2017). Crude fucoidan extracts impair angiogenesis in models relevant for bone regeneration and osteosarcoma via reduction of VEGF and SDF-1. Marine Drugs, 15(6). https://doi.org/10.3390/md15060186

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 17

74%

Researcher 3

13%

Lecturer / Post doc 2

9%

Professor / Associate Prof. 1

4%

Readers' Discipline

Tooltip

Biochemistry, Genetics and Molecular Bi... 11

52%

Agricultural and Biological Sciences 4

19%

Chemistry 3

14%

Medicine and Dentistry 3

14%

Article Metrics

Tooltip
Mentions
Blog Mentions: 1

Save time finding and organizing research with Mendeley

Sign up for free