Role of Pseudomonas aeruginosa DesB in adaptation to osmotic stress

Abstract

Proper membrane fluidity is maintained by adjusting the ratio of saturated fatty acids to unsaturated fatty acids (UFAs), and the control of membrane fluidity plays an important role in bacterial adaptation to stress. Adaptability to these stresses involves survival and colonization of bacteria, thus contributing to bacterial contamination in food. UFAs are synthesized by FabAB-and Des-mediated pathways in Pseudomonas aeruginosa. Therefore, in this study, the roles of UFA-synthesizing proteins of P. aeruginosa in resistance to stresses were examined. The growth and transcription levels of wild-type (WT) P. aeruginosa PAO1 and its mutants were compared. The growth of all strains was inhibited by exposure to 0.5 or 1.0 M sodium chloride, but it was not affected by oxidative or pH stress. In particular, growth was impaired more substantially in the desB mutant than in the WT strain and other mutants, suggesting that DesB has a role in response to salt stress. A comparative transcriptional analysis showed that genes involved in the synthesis of osmoprotectants (trehalose, N-acetylglutaminylglutamine amide, and hydrophilin) were highly expressed in WT P. aeruginosa in response to high salt, but they were rarely expressed in the desB mutant. Furthermore, decreases in osmoprotectant production by the desB mutant were partially complemented by the addition of betaine. These results indicate that P. aeruginosa DesB contributes to adaptation to high salinity by positively regulating the synthesis of osmoprotectants. HIGHLIGHTS desB mutant was sensitive to NaCl compared with the WT and other derivative strains. DesB was closely associated with osmotic stress adaptation. The involvement was via the regulation of osmoprotectant production in P. aeruginosa.