The development of synthetic biology requires rapid batch construction of large gene networks from combinations of smaller units. Despite the availability of computational predictions for well-characterized enzymes, the optimization of most synthetic biology projects requires combinational constructions and tests. A new building-brick-style parallel DNA assembly framework for simple and flexible batch construction is presented here. It is based on robust recombination steps and allows a variety of DNA assembly techniques to be organized for complex constructions (with or without scars). The assembly of five DNA fragments into a host genome was performed as an experimental demonstration. © 2013 Shi et al.
CITATION STYLE
Shi, Z., Wedd, A. G., & Gras, S. L. (2013). Parallel In Vivo DNA Assembly by Recombination: Experimental Demonstration and Theoretical Approaches. PLoS ONE, 8(2). https://doi.org/10.1371/journal.pone.0056854
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