Quercetin regulates the inhibitory effect of monoclonal non-specific suppressor factor β on tumor necrosis factor-α production in LPS-stimulated macrophages

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Abstract

Monoclonal non-specific suppressor factor β (MNSFβ) is a member of the ubiquitin-like family that has been implicated in various biological functions. Previous studies have demonstrated that MNSFβ regulates the ERK1/2-MAPK cascade in the macrophage cell line Raw 264.7. In this study, we found evidence that the flavonol quercetin regulates the effect of MNSFβ on TNFα production in LPS-stimulated Raw264.7 cells. Quercetin inhibited MNSFβ siRNA-mediated enhancement of both TNFα production and ERK1/2 phosphorylation in LPS-stimulated Raw264.7 cells. Quercetin decreased the expression of 33.5-kDa MNSFβ adduct, which is important to the regulation of ERK1/2 activity, in unstimulated Raw264.7 cells. The various flavonoids tested, including other flavonols, did not affect the formation of this adduct. Collectively, MNSFβ and quercetin might share a common pathway in regulating the ERK1/2 pathway in macrophages. This is the first report describing the involvement of flavonoids in the action of ubiquitin-like proteins.

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Nakamura, M., & Omura, S. (2008). Quercetin regulates the inhibitory effect of monoclonal non-specific suppressor factor β on tumor necrosis factor-α production in LPS-stimulated macrophages. Bioscience, Biotechnology and Biochemistry, 72(7), 1915–1920. https://doi.org/10.1271/bbb.80167

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