In this study, a quantum dot (QD) bead -based immunochromatographic assay (QB-ICA) was developed for the rapid, sensitive, and quantitative detection of triazophos. QBs with about 640 times brighter luminescence than the corresponding QDs were used as signal-amplified ICA probes. Under optimum conditions, QB-ICA showed a good dynamic linear detection for triazophos at 0.49–250 ng/mL with a half-maximal inhibitory concentration of 6.97 ng/mL. The average recoveries of intra- and inter assays for triazophos-spiked rice samples ranged from 80.91% to 107.00% with the coefficients of variation below 10%, indicating that QB-ICA had an acceptable accuracy for triazophos detection. The triazophos spiked samples were also detected through gas chromatography, and no significant difference (P > 0.05) was found between the two methods. In summary, the proposed QB-ICA system provided a novel platform for the rapid, sensitive, and quantitative detection of triazophos or other pesticides in food materials.
CITATION STYLE
Wu, Q., Wu, P., Duan, H., Liu, B., Shao, Y., Li, P., … Xiong, Y. (2019). Quantum dot bead-based immunochromatographic assay for the quantitative detection of triazophos. Food and Agricultural Immunology, 30(1), 955–967. https://doi.org/10.1080/09540105.2019.1649638
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