Duodenal Flow of Microbial and Feed Nitrogen in Sheep Fed Normal Soybean Meal or Soybean Meal Treated with Modified Zein

Abstract

The effect of protecting soybean meal from microbial degradation in the rumen on duodenal flow of microbial N and feed N was studied with sheep. The soybean meal was protected with chemically modified zein. Two groups of four wethers, each equipped with a ruminal cannula and a duodenal reentrant cannula, were fed a diet based on corn silage (1 kg of DM/d) that contained either a normal or protected soybean meal supplement. The results showed no appreciable differences between the two supplements in ruminal fluid pH, total N, and NAN concentrations; however, the concentration of total VFA was lower for protected soybean meal than for normal soybean meal. Although the flow of NAN into the duodenum was not affected, bacterial N flow was 18% lower, and feed N flow was 195% higher, for protected soybean meal than for normal soybean meal. The digestibilities of OM, ADF, and N in the digestive tract were not affected by the type of supplement. The treatment of soybean meal decreased the degradability of total feed N in the stomach by 22 percentage points, but the increased supply of feed N into the small intestine because of the treatment was at the expense of decreased bacterial synthesis of protein in the rumen, probably because of a shortage of RDP in the diet.