A highly sensitive SPF10 real-time PCR was developed to achieve simultaneous amplification and detection of the human papillomavirus (HPV) target. That way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. Here, we describe in detail a SYBR Green I-based real-time PCR assay based on SPF10 primers using the LightCycler® 480 system to generate and detect HPV amplicons, which are compatible with the LiPA assay.
CITATION STYLE
Micalessi, M. I., Boulet, G. A., & Bogers, J. (2015). A real-time PCR approach based on SPF10 primers and the INNO-LIPA HPV genotyping extra assay for the detection and typing of human papillomavirus. Methods in Molecular Biology, 1249, 27–35. https://doi.org/10.1007/978-1-4939-2013-6_2
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