Associated vibrio species in shrimp vibriosis from traditional brackish water pond in the north coastal of Central Java, Indonesia

Abstract

Indonesian shrimp cultures are threatened by vibriosis. Some traditional brackish water ponds remained along the north coast of Central Java after the disease outbreaks destroyed the shrimp culture. This study aimed to discover the Vibrio diversity associated with shrimp vibriosis in traditional brackish water ponds. An exploratory method with purposive sampling was used in this study. Twenty-four shrimps presumably infected with vibriosis were collected from two district regions on the north coast of Central Java in July–September 2018. The bacteria associated in shrimp vibriosis were isolated from the telson and inner part of the hepatopancreas with TCBS medium. Forty-one bacteria associated with shrimp vibriosis were obtained, and then repetitive-polymerase chain reaction (rep-PCR) was performed to obtain Vibrio strains. On the basis of rep-PCR results, five respresentative strains were selected for further study. The results of 16S rDNA sequence analysis showed that the JKP03, JKP05, JKP19, JKM01, and JKM06 isolates were closely related to Vibrio rotiferianus, Vibrio diabolicus, Vibrio parahaemolyticus, Vibrio alginolyticus, and Shewanella algae, respectively. Vibrio biodiversity in shrimp vibriosis was high. These results confirmed that traditional shrimp farming was susceptible to vibriosis. Therefore, control methods such as vaccines, probiotics, and immunostimulant formulas must be developed to prevent and control the outbreak of shrimp vibriosis in traditional brackish water pond.