Barley biotechnology requires efficient genetic engineering tools for producing transgenic plants necessary for conducting reverse genetics analyses in breeding and functional genomics research. Agrobacterium-mediated genetic transformation is an important technique for producing barley transgenics with simple low-copy number transgenes. This chapter reports a refined protocol for the systematic high-throughput transformation of the advanced Australian spring barley breeding line WI4330.
CITATION STYLE
Ismagul, A., Mazonka, I., Callegari, C., & Eliby, S. (2014). Agrobacterium-mediated transformation of barley (Hordeum vulgare L.). Methods in Molecular Biology, 1145, 203–211. https://doi.org/10.1007/978-1-4939-0446-4_16
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