Derivação de novas linhagens brasileiras de células-tronco embrionárias humanas sob condições fisiológicas de oxigênio

Abstract

The development and application of human embryonic stem (hES) cells is an important experimental tool for understanding differentiation and development, and offer a wide range of potential applications, including prevention, diagnosis and treatment of human diseases. This area is one of the most promising and fascinating contemporary biology. The essential components of the process of embryonic stem cells derivation are the embryos, embryo manipulation methods, feeder cells, culture media, the oxygen level and the general culture conditions in vitro. In 2008, Pereira’s group generated the first hES cell Brazilian lineage. It is important to continue deriving hES cells lines for both basic research and clinical applications due to loss of genetic and epigenetic stability during hES cells culture and the manipulation. Therefore, the more lineages are generated, we will have a clearer idea about what is happening. In the current study, we has generated two lineages of hES cells, designated BR-4 (46, XY) and BR-5 (46, XX), under physiological oxygen condition. To our knowledge, these are the first reported lineages of hES cells derived in South America under physiological oxygen condition, which was fundamental to the consolidation of this research area in our country to study the epigenetic status of these cells.