Physiology of Insect Cells Cultured in a New Serum-Free Medium

Abstract

The authors report the design of a new serum-free medium for insect cells culture and the physiol. of insect cells in this medium under relevant bioprocess conditions. Sf-9 and High-Five cells were cultured in a stirred tank bioreactor. Various plant protein hydrolyzates were evaluated using a fractional factorial exptl. design. The final medium formulation, called YPR contained: 0.6% yeastolate, 0.5% Primatone RL, 0.1% glucose, 0.022-0.025 % glutamine, 0.1% pluronic F-68 and a lipid mixt. for insect cell culture at 400x diln. This medium is low cost and supports rapid growth and recombinant protein prodn. for both cell lines tested. [on SciFinder(R)]