Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes

38Citations
Citations of this article
60Readers
Mendeley users who have this article in their library.

Abstract

In this work, we evaluate the in vitro response of endothelial cells (EC) to variation in precisely-defined, micrometer to submicrometer scale topography on two different substrate materials, titanium (Ti) and silicon (Si). Both substrates possess identically-patterned surfaces composed of microfabricated, groove-based gratings with groove widths ranging from 0.5 to 50 mm, grating pitch twice the groove width, and groove depth of 1.3 μm. These specific materials are chosen due to their relevance for implantable microdevice applications, while grating-based patterns are chosen for the potential they afford for inducing elongated and aligned cellular morphologies reminiscent of the native endothelium. Using EA926 cells, a human EC variant, we show significant improvement in cellular adhesion, proliferation, morphology, and function with decreasing feature size on patterned Ti substrates. Moreover, we show similar trending on patterned Si substrates, albeit to a lesser extent than on comparably patterned Ti substrates. Collectively, these results suggest promise for sub-micrometer topographic patterning in general, and sub-micrometer patterning of Ti specifically, as a means for enhancing endothelialization and neovascularisation for novel implantable microdevice applications. Copyright:

Cite

CITATION STYLE

APA

Vandrangi, P., Gott, S. C., Kozaka, R., Rodgers, V. G. J., & Rao, M. P. (2014). Comparative endothelial cell response on topographically patterned titanium and silicon substrates with micrometer to sub-micrometer feature sizes. PLoS ONE, 9(10). https://doi.org/10.1371/journal.pone.0111465

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free