The HER2/neu gene (also known as ERBB2) is located on chromosome 17 (q11.2-q12) and encodes a glycoprotein known to be a member of the epidermal growth factor receptor family. Clinically, the determination of its amplification status is of utmost importance, as 10-35% of invasive human breast carcinomas come along with HER2/neu overexpression, and treatment has to be adjusted accordingly. Here a method to analyze HER2 FISH samples with digital microscopy, using the slide scanning -platform Metafer PV (MetaSystems, Altlussheim, Germany), is presented. Metafer PV is a system for the automation of HER2/neu FISH assay analysis of samples hybridized with the Abbott(™) PathVysion(®) probe kit.
CITATION STYLE
Schunck, C., & Mohammad, E. (2011). Automated analysis of FISH-stained HER2/neu samples with Metafer. Methods in Molecular Biology (Clifton, N.J.), 724, 91–103. https://doi.org/10.1007/978-1-61779-055-3_6
Mendeley helps you to discover research relevant for your work.