Autonomous Microfluidic Capillary Network for on Chip Detection of Chemiluminescence

Abstract

This work reports on the design, simulation and fabrication of an autonomous microfluidic network. It is a part of a highly integrated, new analytical platform for the multiparametric detection of bio-organic molecules in extra-terrestrial environment. The proposed microfluidic system, made in SU-8 3050, allows to obtain an autonomous microfluidic network able to have simultaneous capillary filling and fresh solution into each site of detection avoiding cross-contamination among different sites. Computational Fluid Dynamics (CFD) simulations have been carried in order to verify the proper operation of the designed microfluidic network and to optimize it. Technological processes have been refined and adapted in order to ensure good adhesion, using low-temperature and low-pressure bonding avoiding the risk of breaking the glass slides. Experiments have been conducted to verify the autonomous capillary filling of the entire network and its rinsing with buffer solution. The experimental results are in good agreement with the simulations.