The production of N-linked recombinant glycoproteins is possible in a variety of biotechnology host cells, and more recently in the bacterial workhorse, Escherichia coli. This methods chapter will outline the components and procedures needed to produce N-linked glycoproteins in E. coli, utilizing Campylobacter jejuni glycosylation machinery, although other related genes can be used with minimal tweaks to this methodology. To ensure a successful outcome, various methods will be highlighted that can confirm glycoprotein production to a high degree of confidence, including the gold standard of mass spectrometry analysis.
CITATION STYLE
Strutton, B., Jaffé, S. R. P., Pandhal, J., & Wright, P. C. (2017). Generation of recombinant N-linked glycoproteins in E. coli. In Methods in Molecular Biology (Vol. 1586, pp. 233–250). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6887-9_15
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