Opto-Mechanical Coupling in Interfaces under Static and Propagative Conditions and Its Biological Implications

14Citations
Citations of this article
24Readers
Mendeley users who have this article in their library.

Abstract

Fluorescent dyes are vital for studying static and dynamic patterns and pattern formation in cell biology. Emission properties of the dyes incorporated in a biological interface are known to be sensitive to their local environment. We report that the fluorescence intensity of dye molecules embedded in lipid interfaces is indeed a thermodynamic observable of the system. Opto-mechanical coupling of lipid-dye system was measured as a function of the thermodynamic state of the interface. The corresponding state diagrams quantify the thermodynamic coupling between intensity I and lateral pressure π. We further demonstrate that the coupling is conserved upon varying the temperature T. Notably, the observed opto-mechanical coupling is not limited to equilibrium conditions, but also holds for propagating pressure pulses. The non-equilibrium data show, that fluorescence is especially sensitive to dynamic changes in state such as the LE-LC phase transition. We conclude that variations in the thermodynamic state (here π and T, in general pH, membrane potential V, etc also) of lipid membranes are capable of controlling fluorescence intensity. Therefore, interfacial thermodynamic state diagrams of I should be obtained for a proper interpretation of intensity data. © 2013 Shrivastava, Schneider.

Cite

CITATION STYLE

APA

Shrivastava, S., & Schneider, M. F. (2013). Opto-Mechanical Coupling in Interfaces under Static and Propagative Conditions and Its Biological Implications. PLoS ONE, 8(7). https://doi.org/10.1371/journal.pone.0067524

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free