Screening of bovine milk samples for sub-clinical mastitis and antibiogram of bacterial isolates

Abstract

The study was undertaken to find out the incidence of subclinical mastitis (SCM) and to assess the antibiotic sensitivity pattern of the causative organisms in lactating cows in and around Kanakapura taluk, Ramanagara district of Karnataka state. The prevalence of subclinical mastitis was assessed by the results of 3 different screening tests and bacteriological evaluation was done for the milk samples that were found positive. The predominant bacterial isolates recovered were Staphylococcus aureus (58%) and Escherichia coli (23.5%) followed by Staphylococcus epidermidis (8%), Streptococcus sp. (5.5%), Klebsiella sp. (3%) and Bacillus sp. (2%). The in vitro antibiogram studies of bacterial isolates revealed higher sensitivity for ciprofloxacin (89%), ofloxacin (85%), enrofloxacin (82%), gentamicin (80%) and chloramphenicol (75%), resistant to colistin, neomycin, streptomycin, penicillin and tetracycline.