Fluorescent lifetime imaging (FLIM) is a powerful tool for visualizing physiological parameters in vivo. We present here a 3-dye strategy for mapping bioelectric patterns in living Xenopus laevis embryos leveraging the quantitative power of fluorescent lifetime imaging. We discuss a general strategy for disentangling physiological artifacts from true bioelectric signals, a method for dye delivery via transcardial injection, and how to visualize and interpret the fluorescent lifetime of the dyes in vivo.
CITATION STYLE
McMillen, P., & Levin, M. (2024). Optical Estimation of Bioelectric Patterns in Living Embryos. In Methods in Molecular Biology (Vol. 2745, pp. 91–102). Humana Press Inc. https://doi.org/10.1007/978-1-0716-3577-3_6
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