Purification of Japanese encephalitis virus vaccine by zonal centrifugation

Abstract

A large scale procedure for purification and concentration of Japanese encephalitis virus vaccine by a continuous flow isopycnic banding technique in a sucrose density gradient solution, using a K III zonal centrifuge rotor is presented. The quality of zonal purified vaccine was compared with commercial and Japanese National Institutes of Health reference vaccines for antigenicity, immunodiffusion, and allergic encephalitogenicity tests to show its high purity.