The catalytic activity of glass beads, silica gels and anion-exchange resins modified with metal-porphines in the oxidative reaction of NADH

Abstract

Silica gel and glass beads were modified using the acid chloride of metal-tetrakis(4-carboxyphenyl)porphine (M-TCPP) through a peptide bond, and an anion-exchange resin was modified with M-TCPP by electrostatic interaction and physical adsorption. The carriers modified with Co3+-TCPP proved to accelerate the following redox reaction: NADH + O2 carriers modified with Co-TCPP NADH+H2O2. The formation of hydrogen peroxide was confirmed by means of a colored reaction. The glass heads modified with Co3+-TCPP exhibited the strongest activity among the tested carriers, and were expected to be useful practically as a solid catalyst for the formation of hydrogen peroxide from NADH.