Optimization of conjugated linoleic acid production by lactobacillus planetarum

Abstract

Conjugated linoleic acid (CLA), a mixture of positional and geometric isomers of linoleic acid (LA) with conjugated double bonds, has been proved to have a series of physiological functions, including anticarcinogenic activity, antiatherogenic activity, reducing body fat, and modulating immune system. In this paper, we focused on the optimization of culture medium, which is used to convert linoleic acid into CLA by Lactobacillus planetarum. The results showed that the optimal medium was as follows: glucose 2 %, yeast extract 4, MgSO 4·7H2O 0.05, MnSO4·H2O 0.05 CH3COONa 0.2, K2HPO4·3H 2O 0.1 %. In this medium, the yield of CLA has increased to 0.259 g/l, while the transformation rate is 26 %. © Springer-Verlag Berlin Heidelberg 2014.