Kinetic Study of Immobilized Whole-Cell Lipase Rhizopus Oryzae as Biocatalyst in Biodiesel Synthesis through Non-Alcohol Route

Abstract

Utilizing whole-cell biocatalyst is a potential way to reduce catalyst cost in biodiesel production using lipase as catalyst. Whole-cell of Rhizopus oryzae was cultivated by one-step and two-step method and was immobilized on Biomass Support Particles (BSPs) and chitosan-TPP bead. Immobilized whole-cells on BSPs produce 11% (one-step) and 12% (two-step) FAME yield. While, FAME yield produced by immobilized whole-cell in chitosan-TPP beads are 23% (one-step) and 22% (two-step). Kinetic model based Michaelis-Menten used was found to fit fairly the substrate and product concentration profile. Value of K m and V max for R. oryzae whole-cell immobilized on BSP are 4 mole L-1 , 0.05 mole L-1 h-1 (one-step) and 3 mole L-1 , 0.04 mol L-1 h-1 (two-step). While, for immobilized whole-cell in chitosan-TPP bead, the values are 0.3 mole L-1 , 0.01 mole L-1h-1 and 0.2 mole L-1 , 0.008 mole L-1h-1 for single-step and two-step respectively. Index Terms-Biodiesel, immobilization, Michaelis-Menten, whole-cell. NOMENCLATURE F-1 Suspended whole-cell R. oryzae cultivated by single-step cultivation method F-2 Suspended whole-cell R. oryzae cultivated by two-step cultivation method IB-1 Immobilized whole-cell R. oryzae on BSPs cultivated by single-step cultivation method IB-2 Immobilized whole-cell R. oryzae on BSPs cultivated by two-step cultivation method IK-1 Immobilized whole-cell R. oryzae in chitosan-TPP beads cultivated by single-step cultivation method IK-2 Immobilized whole-cell R. oryzae in chitosan-TPP beads cultivated by two-step cultivation method KZ Immobilized commercially C. rugosa lipase on zeolite KK Immobilized commercially C. rugosa lipase in chitosan-TPP beads [P] FAME consentration (mole L-1) [S] Triglyceride concentration (mole L-1) t Time (h) V max Maximum interesterification rate (mole L-1 h-1) K m Michaelis constant (mole L-1) Manuscript