Development of an enzyme-linked immunosorbent assay based on fusion VP2332-452 antigen for detecting antibodies against Aleutian mink disease virus

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Abstract

For detection of Aleutian mink disease virus (AMDV) antibodies, an enzyme-linked immunosorbent assay (ELISA) was developed using the recombinant VP2332-452 protein as an antigen. Counterimmunoelectrophoresis (CIEP) was used as a reference test to compare the results of the ELISA and Western blotting (WB); the specificity and sensitivity of the VP2332-452 ELISA were 97.9% and 97.3%, respectively, which were higher than those of WB. Therefore, this VP2332-452 ELISA may be a preferable method for detecting antibodies against AMDV.

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Chen, X., Song, C., Liu, Y., Qu, L., Liu, D., Zhang, Y., & Liu, M. (2016). Development of an enzyme-linked immunosorbent assay based on fusion VP2332-452 antigen for detecting antibodies against Aleutian mink disease virus. Journal of Clinical Microbiology, 54(2), 439–442. https://doi.org/10.1128/JCM.02625-15

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