Intercellular Adhesion Molecule and Endogenous NOS Inhibitor: Asymmetric Dimethylarginine in Pregnant Women with Gestational Diabetes Mellitus

Abstract

Objective. The aim of the study was to evaluate the concentrations of soluble intercellular adhesion molecule-1 (s-ICAM-1) and endogenous NOS inhibitor, asymmetric dimethylarginine (ADMA), as markers of endothelium dysfunction in patients with gestational diabetes mellitus (GDM). Patients and Methods. The levels of s-ICAM-1 and ADMA were analysed in the group of 56 patients with GDM and compared to 25 healthy pregnant women. The concentrations of s-ICAM-1 and ADMA were measured in serum using ELISA tests. Results. The groups did not differ by baseline descriptors: age (30.75±6.32 versus 28.50±4.95 years, NS) and gestational age (28.96±2.85 versus 29.12±2.96 hbd, NS). The patients with GDM were more obese (BMI 27.93±7.02 versus 22.34±4.21 kg/m2, p=0.032) and had higher concentration of C-reactive protein (6.46±6.03 versus 3.18±3.83 mg/L, p=0.029). In the GDM group the level of ADMA was lower (0.38±0.17 versus 0.60±0.28 μmol/L, p=0.001) and the level of s-ICAM-1 was significantly higher (289.95±118.12 versus 232.56±43.31 ng/mL, p=0.036) compared to controls. Conclusions. The pregnant women with GDM are characterized by higher concentration of s-ICAM-1 that reflects the activation and dysfunction of the endothelial cells. The decreased ADMA level in GDM patients seems to be preventive in the limitation of NO synthesis caused by the impaired insulin action and the endothelial dysfunction.