A method is described for the identification of the antigen recognised by an scFv isolated from an antibody phage display library using selection against a complex mixture of proteins (e.g. intact cells, purified cell surface membranes, and tissue sections). The method takes advantage of a yeast two-hybrid system that additionally allows for reorganization of post-translational modifications to the bait and target proteins. This technique is therefore especially useful for identifying surface-expressed antigens.
CITATION STYLE
Rasmussen, N., & Ditzel, H. J. (2009). Identification of the specificity of isolated phage display single-chain antibodies using yeast two-hybrid screens. Methods in Molecular Biology (Clifton, N.J.), 562, 165–176. https://doi.org/10.1007/978-1-60327-302-2_13
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