Yeast is a powerful model organism for dissecting the temporal stages and choreography of the complex protein machinery during endocytosis. The only known mechanism for endocytosis in yeast is clathrin-mediated endocytosis, even though clathrinindependent endocytic pathways have been described in other eukaryotes. Here, we provide evidence for a clathrin-independent endocytic pathway in yeast. In cells lacking the clathrin-binding adaptor proteins Ent1, Ent2, Yap1801, and Yap1802, we identify a second endocytic pathway that depends on the GTPase Rho1, the downstream formin Bni1, and the Bni1 cofactors Bud6 and Spa2. This second pathway does not require components of the better-studied endocytic pathway, including clathrin and Arp2/3 complex activators. Thus, our results reveal the existence of a second pathway for endocytosis in yeast, which suggests similarities with the RhoAdependent endocytic pathways of mammalian cells. © 2011 Prosser et al.
CITATION STYLE
Prosser, D. C., Drivas, T. G., Maldonado-Báez, L., & Wendland, B. (2011). Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin. Journal of Cell Biology, 195(4), 657–671. https://doi.org/10.1083/jcb.201104045
Mendeley helps you to discover research relevant for your work.