In Vitro Bioactivity Test of Senna Alata (L.) Roxb Leaves Extract

Abstract

Traditionally, Senna Alata (L.) Roxb (Ketepeng Cina) has been used as an alternative medicine for typhus, diabetes, malaria, herpes, eczema, asthma, stomach ulcers, syphilis, influenza, bronchitis, intestinal worms, fungal skin diseases such as tinea versicolor, ringworm and scabies. The study's objective was to determine the active compounds, antioxidant potency and antifungal activity of Senna Alata (L.) Roxb leaves extract. Samples were extracted using the maceration method with 70% ethanol, ethyl acetate and N-hexane as solvents. Phytochemical analysis used the Harbone method, antioxidant testing using 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and antifungal testing using the agar diffusion method. Phytochemical results proved that all Senna Alata (L.) Roxb leaves extract contained alkaloids, saponins, tannins, flavonoids, triterpenoids, steroids, glycosides. While the antioxidant test stated that ethanol and ethyl acetate solvents had solid antioxidant potential with the Inhibition Concentration (IC50) values ​​obtained were 66.01 ppm and 78.23 ppm respectively. While the solvent N-hexane has a moderate antioxidant potential, the IC50 value obtained is 138.80 ppm. The diameter inhibition zones of ethanol, ethyl acetate, and N-hexane extracts against the Candida albicans were 8.66 mm, 10.22 mm and 8.84 mm, respectively. Cryptococcus neoformans was only inhibited by ethyl acetate and N-hexane Senna Alata (L.) Roxb leaves extracts with diameter inhibition zones of 4.84 mm and 4.44 mm, respectively. Key words: Antifungal, Antioxidant, Candida albicans, Cryptococcus neoformans, Phytochemical , Senna alata (L.) Roxb.