Tissue sample preparation for biomarker discovery

Abstract

Global protein expression studies, an approach known as "proteomics," can offer important clues for understanding tumor biology that cannot be obtained by other approaches. Proteomic studies have provided protein expression pro fi les of tumors that can be used to develop novel diagnostic and therapeutic biomarkers. In this chapter, we describe the strategy and design of proteomic studies, as well as the protocols for tissue sample collection and preparation for biomarker discovery, especially tumor biomarkers, followed by a few examples of our recent proteomic studies.