Apoptotic effects of high estradiol concentrations on endometrial glandular cells

Abstract

Context: High serum estradiol (E2) concentrations result in adverse reproductive outcome in in vitro fertilization cycles, and the detrimental effects are probably due to impaired endometrial receptivity. Objective: Endometrial glandular cells (EGCs) are the cells that embryos first interact with during implantation. Our objective is to examine the in vitro EGC alterations after high E2 treatment. Design: This was a prospective study. Setting: The study was conducted at a tertiary university hospital. Patients: Six women in the follicular phase participated in the study. Interventions: EGCs were purified from human endometrium and cultured with different concentrations (0, 10-9, 10-8, 10-7, 10-5, 10 -4 M) of E2. Main Outcome Measure(s): EGC apoptosis and its underlying mechanism were measured. Results: In vitro BeWo spheroid-EGC implantation assay demonstrated that the stimulation with 10-5 and 10-4 M E2 for 2 days decreased embryo implantation potentials. Presence of apoptotic bodies and DNA fragmentation and an increased percentage of sub-G1 phase were found in EGCs treated with high E2 concentrations. The high E2-treated EGCs could be rescued from apoptosis after the addition of estrogen receptor antagonist ICI 182 780. Western blot revealed increased inhibitory-κB (IκB)-expression and decreased nuclear factor-κB (NF-κB) expression in high E 2- treated EGCs, and NF-κB binding site-driven luciferase activity was decreased as well. When EGCs were pretreated with IκB-small interfering RNA, high E2-induced B cell lymphoma 2 (Bcl-2) down-regulation did not occur and EGCs apoptosis was reduced. Bcl-2 overexpression also rescued high E2-induced EGCs from apoptosis. Conclusions: High E2 concentrations induced EGCs apoptosis through enhancing IκB- expression, which in turn suppressed NF-κB expression. The decreased nuclear NF-κB subsequently inhibited Bcl-2 expression and accordingly enhanced EGC apoptosis. © 2014 by the Endocrine Society.