Applications of DNA-stable isotope probing in bioremediation studies.

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Abstract

DNA-stable isotope probing, a method to identify active microorganisms without the prerequisite of cultivation, has been widely applied in the study of microorganisms involved in the degradation of environmental pollutants. Recent advances and technique considerations in applying DNA-SIP in bioremediation are highlighted. A detailed protocol of a DNA-SIP experiment is provided.

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Chen, Y., Vohra, J., & Murrell, J. C. (2010). Applications of DNA-stable isotope probing in bioremediation studies. Methods in Molecular Biology (Clifton, N.J.), 599, 129–139. https://doi.org/10.1007/978-1-60761-439-5_9

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