Nanostructure-initiator mass spectrometry (NIMS) imaging using soft laser desorption/ionization has proven to be a powerful tool in localizing the spatial distribution of intact biomolecules. NIMS specifically has been demonstrated to have high sensitivity and low background, particularly in the low mass range <1,000 Da, making this technique well suited for metabolic imaging studies. Here, we describe NIMS imaging for direct analysis of metabolite composition across a sectioned biospecimen.
CITATION STYLE
Louie, K. B., & Northen, T. R. (2014). Metabolic imaging using nanostructure-initiator mass spectrometry (NIMS). Methods in Molecular Biology, 1198, 313–329. https://doi.org/10.1007/978-1-4939-1258-2_21
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