In this chapter, we describe and discuss our experience of developing high-throughput imaging systems within the Centre for Plant Integrative Biology (CPIB), at the University of Nottingham. After an introductory literature review, a description of the development of robot imaging hardware for high-throughput digital imaging of Arabidopsis thaliana roots inside a growth room is presented. This is followed by a description of the imaging software and techniques that have been developed to analyse such images. The intention of the chapter is to provide an unfamiliar reader with a practical overview of the subject, from the perspective of a team of engineers and computer scientists who have worked together to produce a high-throughput imaging system in active use by plant biologists.
CITATION STYLE
French, A., Wells, D., Everitt, N., & Pridmore, T. (2012). High-throughput quantification of root growth. In Measuring Roots: An Updated Approach (pp. 109–126). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-22067-8_7
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