CD8+ and CD4+ T cells play a critical role in viral clearance during self-limited HCV infection. Moreover, induction of robust and polyfunctional memory T cells is a primary goal in the development of T-cell-based HCV prophylactic vaccines. Direct ex vivo function of HCV-specific T cells without in vitro expansion can be assessed by measuring cytokine secretion or cell proliferation upon in vitro stimulation with HCV antigens using enzyme-linked immunospot (ELISpot) assays, intracellular cytokine staining (ICS) assays, and flow cytometry-based T-cell proliferation assays. In particular, polyfunctionality of T cells can be assessed by ICS assays using multicolor flow cytometry. Here, we provide protocols to measure direct ex vivo functions of HCV-specific T cells.
CITATION STYLE
Park, S. H., & Shin, E. C. (2019). Direct ex vivo functional analysis of HCV-specific T cells. In Methods in Molecular Biology (Vol. 1911, pp. 349–361). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8976-8_24
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