Odorant-regulated ca2+ gradients in rat olfactory neurons

Abstract

Olfactory neurons respond to odors with a change in conductance that mediates an influx of cations including Ca2+. The concomitant increase in [Cai] has been postulated to play a role in the adaptation to maintained odorant stimulation (Kurahashi, T., and T. Shibuya. 1990. Brain Research. 515:261-268. Kramer, R. H., and S. A. Siegelbaum. 1992. Neuron. 9:897-906. Zufall, F., G. M. Shepherd, and S. Firestein. 1991. Proceedings of the Royal Society of London, B. 246:225-230.) We have imaged the distribution of [Cai] in rat olfactory neurons (RON) using the Ca2+ indicator fura-2. A large percentage of the RON (42%, n = 35) responded to odorants with an increase in [Cai]. About half of the responding neurons displayed an increase in [Cai] at the apical end of the cell, but not at the soma. Moreover, in those cells that responded to odors with a standing [Cai] gradient, the gradient could be maintained for long periods of time (minutes) provided that the cells were continuously stimulated. In contrast, K+-induced depolarization elicited a more homogeneous increase in [Cai]. The spatially inhomogeneous increase in [Cai] elicited by odorants in some cells has important implications for the role of Ca2+ in adaptation because channels and enzymes regulated by Ca2+ will be affected differently depending on their location.