Chlorophyll fluorescence photography to detect mutants, chilling injury and heat stress

Abstract

Chlorophyll fluorescence photography with high speed colour film has been used to examine: (i) kinetics of the rise and fall of variable fluorescence in barley leaves, (ii) detection of high fluorescing mendelian and maternally inherited mutants of barley, (iii) detection of protochlorophyllide-deficient and protochlorophyllide-accumulating mutants of barley, (iv) heat stress by photographing the increase of initial fluorescence in heated leaves, and (v) decrease in variable fluorescence in leaves of peanut, tomato and tomato-potato somatic hybrids associated with the development of chilling injury. Filter combinations and a light source for photographing chlorophyll fluorescence are described. The relative intensity and kinetics of the fluorescence from the plants photographed was also measured, so that this could be correlated with fluorescence as detected by photography, and to facilitate the determination of optimal conditions for fluorescence photography. © 1980 Carlsberg Laboratory.