Structural insights into the molecular design of flutolanil derivatives targeted for fumarate respiration of parasite mitochondria

22Citations
Citations of this article
34Readers
Mendeley users who have this article in their library.

Abstract

Recent studies on the respiratory chain of Ascaris suum showed that the mitochondrial NADH-fumarate reductase system composed of complex I, rhodoquinone and complex II plays an important role in the anaerobic energy metabolism of adult A. suum. The system is the major pathway of energy metabolism for adaptation to a hypoxic environment not only in parasitic organisms, but also in some types of human cancer cells. Thus, enzymes of the pathway are potential targets for chemotherapy. We found that flutolanil is an excellent inhibitor for A. suum complex II (IC50 = 0.058 μM) but less effectively inhibits homologous porcine complex II (IC50 = 45.9 μM). In order to account for the specificity of flutolanil to A. suum complex II from the standpoint of structural biology, we determined the crystal structures of A. suum and porcine complex IIs binding flutolanil and its derivative compounds. The structures clearly demonstrated key interactions responsible for its high specificity to A. suum complex II and enabled us to find analogue compounds, which surpass flutolanil in both potency and specificity to A. suum complex II. Structures of complex IIs binding these compounds will be helpful to accelerate structure-based drug design targeted for complex IIs.

Cite

CITATION STYLE

APA

Inaoka, D. K., Shiba, T., Sato, D., Balogun, E. O., Sasaki, T., Nagahama, M., … Harada, S. (2015). Structural insights into the molecular design of flutolanil derivatives targeted for fumarate respiration of parasite mitochondria. International Journal of Molecular Sciences, 16(7), 15287–15308. https://doi.org/10.3390/ijms160715287

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free