Small RNA suppression constitutes one of the major difficulties for a full molecular characterization of their specific roles in plants. Taking advantage of the latest insights into the new post-biogenesis layer of regulation in microRNA (miRNA) activity, it is possible to overcome the above-mentioned limitation (Nat Genet 39:1033–1037, 2007). We engineered the IPS1 non-coding RNA to bear a complementary sequence to a given miRNA family, resulting in specific sequestration of RISC complexes. MIMIC technology allows for the constitutive release of all of the potential targets of a miRNA family as well as tissue-specific and inducible suppression of its activity.
CITATION STYLE
Rubio-Somoza, I., & Manavella, P. A. (2011). Mimicry Technology: Suppressing Small RNA Activity in Plants. In Methods in Molecular Biology (Vol. 732, pp. 131–137). Humana Press Inc. https://doi.org/10.1007/978-1-61779-083-6_10
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