A mechanism for ramified rolling circle amplification

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Abstract

Background: Amplification of single-stranded DNA circles has wide utility for a variety of applications. The two-primer ramified rolling circle amplification (RAM) reaction provides exponential DNA amplification under isothermal conditions, creating a regular laddered series of double-stranded DNA products. However, the molecular mechanism of the RAM reaction remains unexplained.Results: A RAM reaction model predicts exponential accumulation of a double-stranded DNA product size series, and product-size ratios, that are consistent with observed RAM reaction products. The mechanism involves generation of a series of increasing size intermediate templates; those templates produce RAM products and recursively generate smaller intermediate templates. The model allows prediction of the number of rounds of circular template replication. Real-time RAM reaction data are consistent with the model. Analysis of RAM reaction products shows exponential growth limitation consistent with the model's predictions.Conclusions: The model provides a rationale for the observed products of the RAM reaction, and the molecular yield among those products. Experimental results are consistent with the model. © 2010 Beals et al; licensee BioMed Central Ltd.

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APA

Beals, T. P., Smith, J. H., Nietupski, R. M., & Lane, D. J. (2010). A mechanism for ramified rolling circle amplification. BMC Molecular Biology, 11. https://doi.org/10.1186/1471-2199-11-94

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