Gene-Vector Construction

  • Wong D
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Abstract

In the previous chapter, we have discussed various kinds of cloning vectors, which are molecular tools to introduce foreign genes into a variety of host cells. Choosing the right kind of vector to introduce the gene of interest is a key step in cloning. Assuming we intend to express a particular gene in E. coli, here is a description of the process scheme in generating a gene-vector (recombinant {DNA}) construct ready for E. coli transformation.

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Wong, D. W. S. (2018). Gene-Vector Construction. In The ABCs of Gene Cloning (pp. 123–129). Springer International Publishing. https://doi.org/10.1007/978-3-319-77982-9_10

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