The chloride ion is an environmental factor affecting the biosynthesis of pyoluteorin and 2,4-diacetylphloroglucinol in pseudomonas sp. YGJ3

Abstract

The effects of Cl- on the antibiotics productivity of Pseudomonas sp. YGJ3 were investigated. YGJ3 produced pyoluteorin and 2,4-diacetylphloroglucinol, depending on the concentration of Cl- in the culture medium. Cl- stimulated the biosynthesis of pyoluteorin, thereby repressing 2,4-diacetylphloroglucinol biosynthesis. The cell-free extract from the bacteria grown without Cl- showed high activity of monoacetylphlor-oglucinol acetyltransferase, an essential enzyme in 2,4-diacetylphloroglucinol biosynthesis.