Second-generation cobas ampliprep/cobas taqman HCV quantitative test for viral load monitoring: A novel dual-probe assay design

Abstract

HepatitisCvirus (HCV)RNAviral load (VL) monitoring is a well-established diagnostic tool for the management of chronic hepatitis Cpatients.HCVRNAVL results are used to make treatment decisions with the goal of therapy to achieve an undetectable VL result. Therefore, a sensitive assay with high specificity in detecting and accurately quantifyingHCVRNAacross genotypes is critical. Additionally, a lower sample volume requirement is desirable for the laboratory and the patient. This study evaluated the performance characteristics of a second-generation real-time PCR assay, the Cobas AmpliPrep/Cobas TaqManHCVquantitative test, version 2.0 (CAP/CTMHCVtest, v2.0), designed with a novel dual-probe approach and an optimized automated extraction and amplification procedure. The new assay demonstrated a limit of detection and lower limit of quantification of 15 IU/ml across allHCVgenotypes and was linear from 15 to 100,000,000 IU/ml with high accuracy (<0.2-log10 difference) and precision (standard deviation of 0.04 to 0.22 log10). A specificity of 100% was demonstrated with 600 HCV-seronegative specimens without cross-reactivity or interference. Correlation to the Cobas AmpliPrep/Cobas TaqManHCVtest (version 1) was good (n=412 genotype 1 to 6 samples, R2=0.88; R2=0.94 without 105 genotype 4 samples). Paired plasma and serum samples showed similar performance (n=25, R 2=0.99). The sample input volume was reduced from 1 to 0.65 ml in the second version. The CAP/CTMHCVtest, v2.0, demonstrated excellent performance and sensitivity across allHCVgenotypes with a smaller sample volume. The newHCVRNAVL assay has performance characteristics that make it suitable for use with currently available direct-acting antiviral agents. Copyright © 2013, American Society for Microbiology.