Detection of nitrate(NO2-) ions produced in disproportionation of nitrogen(II) oxide in aqueous solution

Abstract

The nitrate ions (NO2-), products of disproportionation of NO in aqueous solution, were detected by an Orion Nitrite Electrode 97-46. Calibrations by means of standard NaNO2 solutions within the range 0.001÷100 ppm indicated linear dependence of EMF on ppm within 1÷100 ppm. Measurements justified the usefulness of this detection method of NO in solutions of OH- concentration lower than 10 -2 mol dm-3 since at higher concentrations the EMF values exceeded the measurement range of the electrode. Occurrence of nitrate ions produced in the disproportionation reaction was additionally confirmed in dependence of OH- concentration by near UV and fluorescence spectra. The calibrated ion-selective nitrate electrode has also been shown, on the basis of Co(II)-dipeptide-OH- systems, as a useful tool in studying reversible NO uptake by Co(II) chelates in aqueous solution. Such a reaction may be regarded as simulating the harmful binding of NO by hemoglobin, where it substitutes the isoelectronic dioxygen.