Antibacterial effect of binahong (Anredera cordifolia (Ten.) Steenis) leaf infusion against black pigmented bacteria

Abstract

Periodontitis is a highly prevalent periodontal disease in Indonesia. Black pigmented bacteria, such as Porphyromonas gingivalis and Prevotella intermedia, are the major pathogens associated with chronic periodontitis. Binahong has been scientifically proven to have an antimicrobial effect. This study investigated the effectiveness of a binahong leave infusion as an antibacterial agent against black pigmented bacteria in vitro. The concentrations of the infusion used in the study were 50%, 65%, 80%, 95%, and 100%. The blank disc diffusion method was performed to measure the zone of inhibition in brucella agar. From the diffusion test on brucella agar, the inhibitory zones were 0.42 mm (50%), 1.21 mm (65%), 1.18 mm (80%), 1.19 mm (95%), and 1.36 (100%). In conclusion, a binahong leaf infusion inhibits the growth of black pigmented bacteria.