Gliomas are the most common malignant primary brain tumors, and new clinical biomarkers and therapeutic targets are imminently required. MicroRNAs (miRNAs) are a novel class of small non-coding RNAs (∼22nt) involved in the regulation of various biological processes. Here, by using real-time polymerase chain reaction, miRNA-132 was found to be significantly deregulated in glioma tissues. Based on the prediction of the target genes of miR-132, we hypothesized that there is a significant association between miR-132 and matrix metalloproteinase (MMP) 16 (MT3-MMP), a protein of the MMP family. We showed that the up-expression of miR-132 inhibited cell migration and invasion in the human glioma cell lines A172, SHG44, and U87. Furthermore, the overexpression of miR-132 reduced the expression of MMP16 in A172, SHG44, and U87 cells. Taken together, our study suggested that miR-132 affects glioma cell migration and invasion by MMP16 and implicates miR-132 as a metastasisinhibiting miRNA in gliomas.
CITATION STYLE
Wang, H., Li, X. T., Wu, C., Wu, Z. W., Li, Y. Y., Yang, T. Q., … Zhou, Y. X. (2015). MiR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro. OncoTargets and Therapy, 8, 3211–3218. https://doi.org/10.2147/OTT.S79282
Mendeley helps you to discover research relevant for your work.