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Virus-induced gene silencing to investigate alkaloid biosynthesis in opium poppy

Humana Press Inc., (2020), 75-92
DOI: 10.1007/978-1-0716-0751-0_7
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Abstract

Virus-induced gene silencing (VIGS) enables the targeted silencing of genes in opium poppy (Papaver somniferum) and has been used extensively to determine or support the physiological functions of benzylisoquinoline alkaloid biosynthetic enzymes. Here we describe detailed protocols involved in the application of VIGS to investigate BIA metabolism in opium poppy.

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Chen, R., Chen, X., Hagel, J. M., & Facchini, P. J. (2020). Virus-induced gene silencing to investigate alkaloid biosynthesis in opium poppy. In Methods in Molecular Biology (Vol. 2172, pp. 75–92). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0751-0_7

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