Metodología Sencilla para Evaluar Bioactividad de Ácidos Húmicos obtenidos de Lignito Mediante Extracción Alcalina y Bacterias Solubilizadoras de Carbón

Abstract

In this work a rapid test to evaluate the bioactivity of humic acids (HA) obtained from a lignite coal through two methods was tested: 1) through chemical extraction with NaOH (HA-Q), and 2) by activity of a bacterial strain (Enterobacter aerogenes). For this, the capacity of the HAs to enhance cellular elongation in coleoptiles of wheat was determined, as well as the ability to induce root proliferation and elongation on pregerminated seeds; AH concentrations between 2,0 and 120 mg C. L-1 were tested with reference to the effect enhanced by an AIA 2,0 mgL-1 solution. It was found that treatment with AH-Q at 40 mg C.L-1 enhance a greater elongation of coleoptiles and roots, whereas a concentration of 20 mg C.L-1 of AH-B enhanced highest bioactivity in all growth parameters evaluated. The results indicate that the evaluated HAs exhibit bioactivity that depends on the type of extraction and on the concentration applied. (English) [ABSTRACT FROM AUTHOR]