Transposable elements can be highly mutagenic because when they transpose they can insert into genes and disrupt their function, a propensity which has been exploited in many organisms to generate tagged mutant alleles. The Mutator (Mu) family transposon is a family of DNA-type transposons in maize with a particularly high duplication frequency, which results in large numbers of new mutations in lineages that carry active Mu elements. Here we describe a rapid and cost-effective Miseq-based Mu transposon profiling pipeline. This method can also be used for identifying flanking sequences of other types of long insertions such as T-DNAs.
CITATION STYLE
Zhang, X., Zhao, M., & Lisch, D. (2020). Cost-effective profiling of mutator transposon insertions in maize by next-generation sequencing. In Methods in Molecular Biology (Vol. 2072, pp. 39–50). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9865-4_5
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