The origin and widespread occurrence of Sli-based self-compatibility in potato

34Citations
Citations of this article
69Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Self-compatible (SC) diploid potatoes allow innovative potato breeding. Therefore, the Sli gene, originally described in S. chacoense, has received much attention. In elite S. tuberosum diploids, spontaneous berry set is occasionally observed. We aimed to map SC from S. tuberosum origin. Two full-sib mapping populations from non-inbred diploids were used. Bulks were composed based on both pollen tube growth and berry set upon selfing. After DNA sequencing of the parents and bulks, we generated k-mer tables. Set algebra and depth filtering were used to identify bulk-specific k-mers. Coupling and repulsion phase k-mers, transmitted from the SC parent, mapped in both populations to the distal end of chromosome 12. Intersection between the k-mers from both populations, in coupling phase with SC, exposed a shared haplotype of approximately 1.5 Mb. Subsequently, we screened read archives of potatoes and wild relatives for k-mers specific to this haplotype. The well-known SC clones US-W4 and RH89-039-16, but surprisingly, also S. chacoense clone M6 were positives. Hence, the S. tuberosum source of SC seems identical to Sli. Furthermore, the candidate region drastically reduced to 333 kb. Haplotype-specific KASP markers were designed and validated on a panel of diploid clones including another renown SC dihaploid G254. Interestingly, k-mers specific to the SC haplotype were common in tetraploid varieties. Pedigree information suggests that the SC haplotype was introduced into tetraploid varieties via the founder “Rough Purple Chili”. We show that Sli is surprisingly widespread and indigenous to the cultivated gene pool of potato.

References Powered by Scopus

Trimmomatic: A flexible trimmer for Illumina sequence data

42537Citations
N/AReaders
Get full text

Matplotlib: A 2D graphics environment

25067Citations
N/AReaders
Get full text

BEDTools: A flexible suite of utilities for comparing genomic features

16533Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Genome design of hybrid potato

134Citations
N/AReaders
Get full text

A nonS-locus F-box gene breaks self-incompatibility in diploid potatoes

62Citations
N/AReaders
Get full text

Neofunctionalisation of the Sli gene leads to self-compatibility and facilitates precision breeding in potato

61Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Clot, C. R., Polzer, C., Prodhomme, C., Schuit, C., Engelen, C. J. M., Hutten, R. C. B., & van Eck, H. J. (2020). The origin and widespread occurrence of Sli-based self-compatibility in potato. Theoretical and Applied Genetics, 133(9), 2713–2728. https://doi.org/10.1007/s00122-020-03627-8

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 26

67%

Researcher 8

21%

Professor / Associate Prof. 3

8%

Lecturer / Post doc 2

5%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 30

77%

Biochemistry, Genetics and Molecular Bi... 7

18%

Social Sciences 1

3%

Earth and Planetary Sciences 1

3%

Save time finding and organizing research with Mendeley

Sign up for free