Numerous technological improvements, including progress in vector design, simplification of virus isolation techniques, and advancements in molecular biology and cell culture technologies, have greatly facilitated the use of the baculovirus-insect cell system for routine production of recombinant proteins. This chapter outlines the basic techniques for small-scale protein production using the Baculovirus Expression Vector System (BEVS), including protocols for titer estimation in 96-well plates, expression optimization in 24-well plates, and recombinant protein expression from adherent and suspension cultures in six-well plates and in 50 mL insect cell cultures.
CITATION STYLE
Yang, J. P. (2016). Small-scale production of recombinant proteins using the baculovirus expression vector system. In Methods in Molecular Biology (Vol. 1350, pp. 225–239). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3043-2_10
Mendeley helps you to discover research relevant for your work.