Isolation and structural characterization of rhamnogalacturonan ll-borate complex from Pinus densiflora

15Citations
Citations of this article
11Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

A boron-containing pectic polysaccharide was isolated from Driselase digests of akamatsu (Pinus densiflora) cell wall. The boron-containing polysaccharide was purified by successive column chromatography of Cosmosil C18 OPN, DEAE Sepharose FF, and Superdex 75. The complex had 0.12% (w/w) boron (B) and 11B-NMR spectroscopy showed the complex to be the tetrahedral diester form of boron. The sugar composition and glycosyl linkage analyses showed that the sugar portion of the complex was rhamnogalacturonan-II (RG-II). The boron-attached glycosyl residue was identified to be 2-O-Me-xylose-containing apiosyl side chain in RG-II. These results established that the location of borate in the cell wall is conserved among dicots, gramineous monocots, and coniferous gymnosperm. © The Japan Wood Research Society 1999.

Cite

CITATION STYLE

APA

Shimokawa, T., Ishii, T., & Matsunaga, T. (1999). Isolation and structural characterization of rhamnogalacturonan ll-borate complex from Pinus densiflora. Journal of Wood Science, 45(5), 435–439. https://doi.org/10.1007/BF01177918

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free